Evaluation of kinetic and thermodynamic parameters of Rizatriptan using nano-biosensor

In this research, Nano-graphene (NG), bovine serum albumin (BSA) and agar powder were used as electrocatalyst, selective biological agent and supporting material in construction of novelRizatriptan benzoate (RB) nanobiosensor, respectively [1,2]. Experimental parameters for RB determination, such as BSA/NG ratio, potential scan rate, pH and supporting electrolyte have been optimized. This optimized nanobiosensor (NBRNBS) was applied for electrochemicalassay of RB in phosphate buffer solution (PBS, pH = 7) using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Under the optimum condition, RB could be linearly detected in the acceptable concentration range of 1.0 × 10-3 M to 1.0 × 10-4 M (R2 = 0.9946). Thedetection limit was down to around 7.5 × 10-5 M and maximum current response was obtained at pH=7. Kinetic and thermodynamic parameters such as the standard rate constant, diffusion coefficient, activation energy and Gibbs free energy were evaluated [3]. The MBRNBS exhibits a very good performance for detection of RB in the presence of Sumatriptan, Propranolol and Ergotamine in pharmaceutical preparations.

Keywords:

Rizatriptan , Bovine Serum Albumin , Nano-graphene , Nano-biosensor , Cyclic voltammetry , Kinetic and Thermodynamic parameters