A morphological study of porous gelatin/chitosan scaffolds prepared by freeze drying

Freeze-drying is extensively used for fabrication of porous materials in tissue engineering and biomedical applications, due to its versatility and use of no toxic solvent. However, it has some significant drawbacks. Conventional freeze-drying technique lead to the production of heterogeneous porous structure with side orientated columnar pores. To achieve a scaffold with desirable microstructure for skin tissue engineering, we modified freezing step by controlling the rate of cooling and regulation of heat transfer across the sample. It could create a homogeneous porous structure with more equiaxed non-oriented pores

Keywords:

Gelatin , Freeze-drying , Microstructure , Tissue engineering