Optimization of Polysaccharide Extracted from theManna by Ultrasound

In this study, the technology of ultrasound waves are used to extract polysaccharides from manna plant to enhance the extraction efficiency. Independent variables examined including extraction temperature, time of the waves and the ratio of solvent to dry matter were employed at three levels of response surface methodology and Box-Behnken design to optimize and studies of linear effects and interaction of process variables on polysaccharide extraction efficiency. Results showed that three variables selected have significant effect on the extraction yield of polysaccharides. Polysaccharide extraction efficiency increased withincreasing extraction temperature, time of the waves and the ratio of solvent to dry matter. The optimum conditions for extraction is included extraction temperature 70 ° C, extraction time 10 min and the ratio of solvent to dry matter 20 ml per gram. Under these conditions, the extraction efficiency was obtained 15.2394% and well matched with the models predicted.

Keywords:

manna , extraction , polysaccharides , optimization , response surfacemethodology (RSM)