The effect of cyclic Carnosine peptide analogues on IsolatedMitochondria of A549 Cells

Our study showed that a various range of toxicity on A549 cells was resulted by the cyclic Carnosine analoguesusing MTT assay. Also, applying peptides on the mitochondria of A549 cells, a raise of mitochondrial reactive oxygenspecies (ROS) level, mitochondrial membrane potential (Δψm) collapse of the affected mitochondria were detected.Based on the overall results, cyclic Carnosine analogues, especially compounds 2c [Cyclo-(Pro-β-alanine-His-β-alanine-His)], 4c [Cyclo-(Pro-His-β-alanine-β-alanine-His)] and 6c [Cyclo-(Pro-β-alanine-His-His-β-alanine)], showedmore toxic activity than other Carnosine analogues, which would be encouraging to develop these peptide analogues asnew anticancer and complementary therapeutic agents for the treatment of lung cancer.

Keywords:

Carnosine , Cytotoxicity , Apoptosis , Mitochondria