In vitro cytotoxic effects of green zno nanoparticles prepared from the origanum majorana extract by green method

Nanotechnology refers to the synthesis and manipulation of nanoscale materials, which provides the possibility of changing the physical and chemical properties and biological interactions of nanoparticles.Metal oxides are classified in the group of inorganic nanoparticles that have shown promising applications in the fields of medicine, drug delivery, imaging, cancer therapy, and so on. Zinc oxide nanoparticles are in the metal oxide group that have unique chemical, physical and biological properties. Recently, Zno nanoparticles have attracted a lot of attention in the treatment of cancer andmany studies have shown its effectiveness in inhibiting cancer cells. On the other hand, one of the considerations is to evaluate nanoparticle effects on normal cells. Since treatments should not havedeleterious effects on normal cells, such studies are important. Therefore, in this study, the effect of zinc nanoparticle toxicity on cancer (Hep G2) and normal cells (Huvec) was evaluated. Two types ofcells including HepG2 as a cancer and Huvec as an normal cells were used to investigate the survival cell against ZnO NP exposure. For this purpose, seeded cells in a 96 well plate were exposed tovarious concentrations of zinc nanoparticles for 24h. After treatment period, the treatment medium was drained and the cells were exposed to MTT solution. After 4 h of incubation in the dark, the MTTwas evacuated and the cells were exposed to DMSO. Then, the absorbance was measured using ELISA reader. The amount of nanoparticle toxicity on cells showed that the nanoparticle was able to inhibitcancer cells (IC50: 15 μg/ml) with higher potency and lower concentrations than normal cells (IC50: 80μg/ml). The obtained result allows the use of this nanoparticle to eliminate cancer cells in living tissue because the Zn O nanoparticle is able to eliminate cancer cells in a particular concentration, but does not affect normal cells. The study of the cytotoxic effect of nanoparticle on cancer cells is one ofthe most important parts of the study, but the effect of such compounds on normal cells compared to cancer cells can provide an appropriate insight into the use of these compounds

Keywords:

Cancer Prevention , Cell and Cancer , Nutrition and Cancer , Cancer Treatment and Management , Drugs and Cancer