Biosynthesized cerium oxide nanoparticles induced cell death in mda-mb- 231 breast cancer cell line

Cancer is one of the deadliest diseases in the world, but no definitive treatment has yet been discovered for it. There are various therapies for preventing cancer progression, each of them has its own special side effects. Today, many researchers have focused on the use of green nanoparticles to treat cancer. Data on biomedical applications of cerium oxide nanoparticles (CeO2-NPs) are promising, yetinformation regarding their action in cancer cells is incomplete and there are conflicting reports about in vitro cytotoxicity. But many reports indicate low toxicity and high antioxidant potency of these nanoparticles. The purpose of this study was to examine the cytotoxic effects of CeO2-NPs biosynthesized with Hyssopus officinalis extract (CeO2-NPs/Hf) on MDA-MB-231 breast cancer cells.MTT is one of the most widely used methods for testing the toxicity of materials on different cells. In this study, different concentrations of CeO2-NPs/Hf (0, 62.5, 125, 250 and 500μg/ml) were preparedand then added to the MDA-MB-231 cells grown in 96 well plates. After 24, 48 and 72 h of exposure,all treatment medium were evacuated and then the cell survival was measured using MTT protocol. The results of the MTT assay showed that CeO2-NPs/Hf decreased the proliferation of MDA-MB- 231cells in dose- and time-dependent manner. The results showed that at 24, 48 and 72 h aftertreatment, the CeO2-NPs/Hf in all concentrations tested by the MTT assay showed a significant difference (p ≤ 0.001) with the control group and were able to inhibit growth and induction of cellcytotoxicity on cancer cells with IC50 about 400, 350 and 200 μg/ml. Cerium oxide nanoparticles biosynthesized By Hyssopus officinalis extract have the ability to induce apoptosis in MDA-MB-231.Thus, they could prepare the way for further research on the design of green therapeutic agents for the treatment of breast cancer

Keywords:

Breast Cancer , Cancer Prevention , Cell and Cancer , Multidisciplinary Cancer Research , Cancer Treatment and Management