Anticancer effects of green synthesis of zno nanoparticles using rubia tinctorum extract

Nanotechnology allows the manipulation of substances at the nanoscale level. Inorganic nanomaterials such as ZnO nanoparticles synthesized by chemical methods have the disadvantage of using hazardous materials. However, green chemistry allows us to synthesis nanoparticles avoiding the use of these harmful materials. The goal of this study is to synthesis ZnO nanoparticles using green chemistry, which has no trace of toxic chemicals. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay was performed on colon cancer cell line. This colorimetric test is based on the resuscitation of tetrazolium salt by the succinate dehydrogenase of living cells and the formation of formazan crystals. The MDA-MB-231 cancer cells were incubated 24, 48, and 72 hours with a different concentration of ZnO nanoparticles including 15, 32, 62, 125 and 250 μg/ml compared cell without treatment as a control. After given times the treatments were removed and MTT was added to the wells. In order to solve tetrazolium DMSO was added to the wells and optical absorbance was read at 570 nm. The results showed that ZnO nanoparticles kill cancer cells in time-dependent andconcentration-dependent manner. However, the values of IC50 was determined about the 160, 126 and 67 μg/ml for 24, 48 and 72 hour-treatments, respectively. the results showed the high potential of ZnOnanoparticels synthesised with Rubia tinctorum extract in inhibition of cancer cells growth. However, more in vitro studies should perform in order to confirm these effects

Keywords:

Breast Cancer , Cell and Cancer , Cancer Risks , Cancer Treatment and Management , Drugs and Cancer