Isolation and Sequence Analysis of GpdII Promoter of the White Button Mushroom (Agaricus bisporus) from Strains Holland737 and IM008
Publish Year: 1394
نوع سند: مقاله ژورنالی
زبان: English
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JR_IJHST-2-1_004
تاریخ نمایه سازی: 21 فروردین 1397
Abstract:
Many recent studies have shown that glycosylation patterns of Agaricus bisporus are similar to those of mammalians, so that this organism is a good candidate for the expression of glycosylated pharmaceutical protein. To achieve constant interested gene expression in all cells of the organism, proper promoter isolation is necessary. To isolate this promoter, PCR with specific primers was performed on extracted DNA of the white button mushroom strains Holland737 and IM008. The PCR amplified 290 bp fragments of gpdII promoters. IM008 gpdII promoter was used to construct pCAMBIAH8 plasmid. Comparison of isolated promoters among sequence records at NCBI demonstrated high similarity between IM008 gpdII promoter and previously reported gpdII promoter. Sequence analysis of isolated promoters revealed several point mutations on this promoter. TACAAA promoter sequence in −65 site acts as TATA box. Among the three CAAT candidate sequences, one is functional, which is located at position −108. Transformation of the white button mushroom with constructed pCAMBIAH8 plasmid was successfully performed
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Authors
mohsen ashrafi
Department of Agronomy and Plant Breeding, College of Agriculture, University of Zanjan, Zanjan, Iran
mohammad farsi
Department of Plant Breeding and Plant Biotechnology, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
amin mirshamsi
Department of Plant Breeding and Plant Biotechnology, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
mozhgan parvandi
Department of Plant Breeding and Plant Biotechnology, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran