14-bp insertion/deletion polymorphism of the HLA-G gene in breast cancer among women from north western Iran

Introduction: The human leukocyte antigen-G (HLA-G) gene plays a vital role in the suppression of immune responses. Recently, a number of studies have reported an association between HLA-G and diseases. Some of the studies have revealed that the 14-bp insertion/deletion polymorphism might be associated with various diseases. In this study, we have analysis genetic association between HLA-G 14bp deletion/Insertion polymorphism with breast cancer among North Western-Iranian Women.Methods: Frequency of HLA-G 14bp deletion polymorphism were determined in 100 women affected to breast cancer , in addition, 100 age-sex and ethnically matched healthy individuals participated in this study as the control group. Genotyping was done using polymerase chain reaction and electrophoresis assays. The data were compiled according to the genotype and allele frequencies, which were compared using Fisher’s exact test. It was set statistical significance at P<0.05 for tests.Results: The result showed that it had a lower frequency of HLA-G 14bp deletion at allelic and genotype levels and revealed statistically significant associations with breast cancer cases when compared with the controls. Allele frequency was 42% and 58% among cases and controls group respectively, and the difference were statistically significant (P-value = 0.034, OR = 1.907; 95% CI=1.047– 3.481).Conclusions: Our data support a possible susceptibility trend that HLA-G 14bp insertion polymorphism may be a potential genetic risk factor for breast cancer, but future studies are required to establish the exact role of this polymorphism for susceptibility to breast cancer.

Keywords:

Breast cancer , HLA-G , Polymorphism , Northwestern Iranian Women