Effect of hydro alcoholic extract of Nigella sativa on Caspase3 gene expression in MCF7 cell line
Publish place: 10th International Breast Cancer Congress
Publish Year: 1393
نوع سند: مقاله کنفرانسی
زبان: English
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ICBCMED10_060
تاریخ نمایه سازی: 21 اردیبهشت 1397
Abstract:
There are problems such as treatment failure, drug resistance, heavy costs and other problems related to cancer treatment, which cause the plants to attract the interest of many researchers, because they have no side effects. In this study effect of Nigella Sativa on, caspase-3 gene expression,one of genes inducing of apapotosis was studied. First Nigella sativa was extracted by 70% ethanol and concentrations of 200 and 400 μg/ml were prepared and MCF-7 cell lines treated by extractions. Then cells harvested on 24, 48 and 72 hours after treatment. Expression of caspase-3 was evaluated by Real-time PCR .The results showed that caspase-3 gene expression in MCF-7 cells was not changed significantly by Nigella sativa extract. As the concentrations of extract used in this study had no effect on caspase-3 gene, it seems that other concentrations of Nigella sativa was effective or other genes in apoptosis pathway were affected.
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Authors
Fathemeh Moshtaghi
MSc student, Department of Biology, Faculty of Basic Sciences, University of Zabol, Zabol, Iran
Sedigheh Esmaeilzadeh Bahabadi
Assistant professor, Department of Biology, Faculty of Basic Sciences, University of Zabol, Zabol, Iran
Mahta Mazaheri Naeini
Assistant professor, Department of Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical science, Yazd, Iran
Shahla Najafi
Assistant professor, Department of Biology, Faculty of Basic Sciences, University of Zabol, Zabol, Iran