Construction of an Oncolytic Adenovirus for Selective Replication in Breast Cancer Cells
Publish place: 12th International Congress on Breast Cancer
Publish Year: 1394
نوع سند: مقاله کنفرانسی
زبان: English
View: 478
نسخه کامل این Paper ارائه نشده است و در دسترس نمی باشد
- Certificate
- من نویسنده این مقاله هستم
این Paper در بخشهای موضوعی زیر دسته بندی شده است:
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
ICBCMED12_052
تاریخ نمایه سازی: 2 تیر 1397
Abstract:
Introduction & Aim: Targeted therapy of cancer using oncolytic viruses is a new advanced treatment. In this study we used microRNA-targeting strategy to generate a new adenovirus that selectively replicate in breast cancer cells but spare normal cells. MicroRNA-145 is reportedly down-regulated in breast tumor cell lines and malignant tissues but is frequent in normal cells. We constructed a miRNA-145- regulated oncolytic adenovirus. This study is the first effort for targeting oncolytic adenovirus to breast cancer cells using differential expression of miRNA in breast tumor and normal cells. Methods: A control adenovirus and an adenovirus carrying miR-145-5p targets in E1A gene (AD5-miR- 145-5pT) generated by Gateway technology. The MCF-7 human breast cancer cell line and the normal human mammary epithelial cells (HMEpC) were infected with AD5-control and AD5-miR145-5pT, and then the viral titers were measured 12, 24, 36 and 48 h post-infection using TCID50 assay. MTT assay was performed to compare the ability of adenoviruses for destroying MCF-7 cells. Results: Growth kinetic analysis of AD5-miR-145-5pT in MCF-7 cells and HMEpC showed that replication of the adenovirus was inhibited in HMEpC as normal breast cells, whereas the virus efficiently replicated in MCF-7 cells. Infectious titer of AD5-miR-145-5pT at 48 h post-infection in HMEpC was 1600fold lower than that of the AD5-control. MTT assay showed that ability of AD5-miR-145-5pT for destroying the MCF-7 cells is similar to AD5-control Conclusion: The use of differential expression pattern of miR-145 in breast cells is a good mechanism for targeting of adenovirus toward breast cancer cells
Authors
Mohammad Shayestehpour
Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Shaghayegh Yazdani
Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Talat Mokhtari-Azad
Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Jila Yavarian
Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran