ESBL PREVALENCE IN ENTROBACTERIACEAE ISOLATED FROM HOSPITAL’S WASTEWATER IN KARAJ

Background and Aim:Hospitals are hotspots for antimicrobial-resistant bacteria (ARB) and will be ejected from hospitals via wastewater systems. In this study, we present AmpC and ESBL prevalence in Entrobacteriaceae strains isolated from hospitals wastewater in Karaj.Methods:A total of 100 Entrobacteriaceae strains isolated from hospital’s wastewater in Alborz province during the summer of 2018. Bacterial strains were identified by standard microbiological and biochemical tests. The antimicrobial susceptibility test was determined according to Kirby Baur assay. ESBLs producers were screened by phenotypic confirmatory test (PCT).Results:Among the organisms cultured, Escherichia coli (74%) was the most common organism followed by Klebsiella oxytoka (7%). Antibiotic resistance pattern were observed as follows: cefotaxim 13%, ceftriaxone 19%, ceftazidim 15% and cefepim 7%. The ratio of isolates was detected as ESBLs and AmpC producers were 17% and 23%, respectively.Conclusion:Antimicrobial resistance should now be seen as an ‘environmental pollutant’, and new wastewater treatment processes must be assessed for their capability in eliminating ARB, especially from hospital effluents.

Keywords:

ESBL , Entrobacteriaceae , wast