STUDY OF BACTERIAL CONTAMINATION IN GALLBLADDER OF SHEEP SLAUGHTERED IN SHIRAZ ABATTOIR

Background and Aim:Generally gallbladder is a sterile organ, but pathogenic microorganisms can reside transiently or permanently in the gallbladder. The aim of this study was to isolate and characterize the bacterial contamination present in bile and gallbladder of sheep slaughtered in slaughter house in Shiraz, Fars province.Methods:From April to May 2018 from 26 samples were taken by syringe from gallbladder at the slaughter house for isolation and identification of bacterial contamination. All samples were transferred to the laboratory of microbiology, faculty of veterinary medicine, Shiraz University, in cold condition in about 2 hr. in laboratory bile samples inoculated on Blood and MacConkey agar plates and incubated at 37C for 24-48hr. then from colonies were grown prepared smear and gram stained. Then according to morphological and biochemical traits bacterial isolates characterized.Results:From 26 bile samples 8 samples did not growth (30.81%). But in 18 samples after morphological and biochemical evaluation different bacteria were isolated. According to our finding percentage of bacteria in bile cultures were Staphylococcus spp. 19.23%, Salmonella spp. 15.38%, Escherichia coli 11.53%, Neisseria 11.53%, Micrococcus 3.8%, Seratia 3.8%, Yersinia 3.8%.Conclusion:According to different studies factors such as fasciolosis, hepatitis and Cholelithiasis can cause chronic inflammation of the gallbladder. In my opinion these situations can act as a predisposing factor for entrance of some bacteria into the gallbladder.but it is not likely to become clinically significant.

Keywords:

Gallbladder , Bacterial , Slaughterhouse , Shiraz