DETERMINATION OF TOTAL PROTEIN CONTENT IN BACTERIAL SUSPENSION CONTAINING CLOSTRIDIUM PERFRINGENS TYPE D

Background and Aim:Clostridium perfringens (C. perfringens) secretes at least 17 types of toxins. C. perfringens type D, in addition to secreting major toxins like alpha and epsilon, also produces various alternative toxins. C. perfringens type D, due to the secretion of these toxins, can cause enterotoxemia in domestic livestock and a lot of economic losses to the animal husbandry industry. Accordingly, the study of the amount of secreted toxin, which has the protein nature, is of particular importance.Methods:For this purpose, at first C. perfringens type D cultured in a nutrient medium in anaerobic conditions then maintained in the incubator so the suspensions that containing toxin were prepared. After centrifuge and preparing various dilution, spectrophotometer was used to measure total protein content in two wavelengths.Results:The mean amount of toxins that produced from C. perfringens type D isolates was 7.1 mg/ml.Conclusion:The total protein content obtained from our samples was the significant difference with standard strain.

Keywords:

Clostridium perfringens , Toxin , Culture , Total Protein