Electrochemical Determination of the Anticancer Drug Amsacrine at a ds-DNAModified Graphene Carbon Paste Electrode and its Application as a Label-FreeElectrochemical Biosensor

DNA has a key role in the process of life as heritage data are carried with it. DNA is an important target for smaller molecules such as medicines, metals, and carcinogens [1].The interaction between several anticancer medicines with DNA has been investigated using different methods. Although, these methods are time-consuming and expensive and need more materials, Recently, the electrochemical investigation of interaction between anticancer drugs and DNA has been increased. Among these methods, DNA electrochemical biosensors are one of the main groups [2]. Amsacrine is a well-known anticancer agent with a good activity against refractory acute leukemias as well as Hodgkin’s and non-Hodgkin’s lymphomas [3]. In current research, based on the interaction between dsDNA and amsacrine, amsacrine was determinedby a simple, fast, reliable, and sensitive DNA biosensor. After optimizing the main experimental parameters, This DNA-coated GPE was fabricated to evaluate its performance analytically. A linear dynamic range of 7.0×10-7– 10×10-4 M was obtained for amsacrine.Ultimately, the proposed biosensor was found to be capable of successfully analyzing human serum and urine samples.

Keywords:

Amsacrine , Graphene , Carbon paste electrode , DNA