Study of Interactions between Cholinium L-alaninate as a Green Entrianer and Bovine Serum aAlbumin in Water Medium by Measuring of VolumetricProperties and UV-Visible Spectra

Cholinium amino acids ([Ch][AA]) are novel, low toxic and biocompatible ionic liquids(ILs) which are used widely in separation processes[1-3]. Since, the most of these processes weredone in an aqueous media, it is vital to achieve better understanding of the interactions betweenILs with proteins in this media [4, 5]. In this respect, in this work, we focused our attention on theinvestigation of physicochemical properties of the cholinium L-alaninate ([Ch][L-Ala]) in waterand in aqueous bovine serum albumin (BSA) solution with different mass fractions 0.050 to0.150 at T = 298.15 K under atmospheric pressure (≈ 85 kPa). Density (  ), speed of sound (u)and UV-visible spectra of the studied aqueous solutions were experimentally measured. Theobtained values from the density and acoustic studies were used for computing the limitingvalues for apparent molar volume ( 0 V ), apparent molar isentropic compressibility ( 0  ), and alsothe transfer molar volume (Δtran0 V ) and isentropic compressibility (Δtran0  ) of [Ch][L-Ala] fromwater to aqueous BSA solutions. Finally, the BSA-[Ch][L-Ala] interactions in the investigatedsolutions were evaluated on the basis of changing the above thermodynamic properties fromaqueous binary to ternary solutions consist of the [Ch][L-Ala] and BSA.

Keywords:

Cholinium L-Alaninate , BSA , UV-Visible Spectra , Transfer Molar Volume , Transfer Isentropic Compressibility