A novel enzymatic biosensor for the determination of Methanol
Publish place: The 2nd International Congress and the 25th National Congress of Food Science and Technology
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
NCFOODI25_039
تاریخ نمایه سازی: 26 خرداد 1398
Abstract:
Methanol is a serious toxic component for humans which can cause permanent blindness by destruction of the optic nerve. An electrochemical enzyme-based biosensor plane has been designed by the self-assembly technique for determination of methanol in herbal distillates samples, which was composed of self-assembled thiodibutyric acid monolayer and Alcohol oxidase and peroxidase enzymes. The electrochemical methanol biosensor exhibited a linear relationship between target concentration and oxidation current in the range of 25-400 ppm and its detection limit was 10 ppm. We also measured methanol content in several commercially available herbal distillates samples with the biosensor. The accuracy of results was verified by using GC measurements, which showed good agreement with results from proposed biosensor.
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Authors
Asma Verdian
Department of Food Nanotechnology, Research Institute of Food Science and Technology (RIFST), Mashhad, Iran
Zahra Arefi
Medical Toxicology Research Center, Pharmacy School, Mashhad University of Medical Sciences, Mashhad, Iran