High performance osteogenic differentiation of human adipose tissue derived mesenchymal stem cells on polyurethane nanocomposite scaffolds containing modified clay nanoplates invitro

IntroductionTissue engineering as the alternative strategy provide an opportunity to regenerative large bone tissue lesions. The use of biodegradable scaffolds with stem cells, cytokines and growth factors improves cell survival and treats the affected area to a great extent. ObjectivesIn the present study, polyurethane (PU) scaffolds containing modified clay nanoplates (MCNPs) were synthesized and used to high performance differentiation of human adipose tissue derived mesenchymal stem cells (hADSCs) into osteogenic cells.MethodsOsteogenic differentiation of hADSCs on MCNPs and PU scaffolds were investigated by osteogenic markers such asalkaline phosphatase, calcium content and Alizarin Red staining on days 7 and 14 after osteogenic induction.ResultsOur results showed the higher ALP enzyme activity and more calcium content of differentiated hADSCs on PU-MCNPs. ConclusionIn conclusion, based on obtained results the use of synthesized PU-MCNPs scaffolds by providing 3-D space benefits for cells proliferation and effective osteogenic differentiation suggests for tissue engineering and repair of bone defects

Keywords:

Osteogenic differentiation , Mesenchymal stem cells , Polyurethane nanocomposite scaffolds , Clay nanoplates