Evaluationof the effects of chitosan hydrogels with nanocellulose containing erythromycin on histological parameters and gene expression of bfgf andhif-1 in skin wound healing in male rats

IntroductionWound healing is one of the medical problems and costs to the community every year. Chitosan is one of the best natural biopolymers that can effectively reduce the repair time in combination with other natural or synthetic polymers. The aim of this study was to investigate the effect of chitosan nanocellulose hydrogel with erythromycin antibiotics on expression of bFGF and hHIF-1 genes and histological parameters in rat skin ulcers.MethodsThis study was performed on 75 male wistar rats with 1.5 to 1.5 cm incisors in the back of the neck. The rats were randomly assigned to chitosan, chitosan nanocellulos, chitosan nanocellulos with erythromycin and erythromycin and control group. The rats were taken on the 4th, 7th, 14th days of the treatment and adjacent healthy skin samples were evaluated for histological studies by stereology and examination of the expression of bFGF and Hif-1 genes by Real time PCR method.ResultsThe results showed that chitosan nanocellulose hydrogel with erythromycin can significantly decrease neutrophil inflammatory cells on days 4 and 7 than other groups. Chitosan hydrogel nanocellulose group with erythromycin and without erythromycin was able to increase epidermis, dermal and fibrosis volume significantly more than other groups. The expression of bFGF andHIF-1 genes in chitosan nanocellulose hydrogel group with erythromycin and chitosan hydrogel group with nanocellulose were significantly higher in comparison with other groups on days 7 and 14.ConclusionOverall, the results showed that chitosan hydrogel with erythromycin containing nanocellulose could significantly improve wound healing in compare with other groups.

Keywords:

Chitosan , Erythromycin , Hydrogel , Wound healing