Poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) micelles for controlled release of all-trans-retinoic acid; in vitro characterization
Publish place: 1st International Iranian Tissue Engineering and Regenerative Medicine Congress (ITERM2018)
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
ITERMED01_132
تاریخ نمایه سازی: 7 مرداد 1398
Abstract:
In order to achieve the controlled release of all-trans-retinoic acid (ATRA), poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL) copolymer with average molecular weight of 5.34 kDa was synthesized. The nanosized micelles were prepared from copolymer by nano-precipitation method. Critical association concentration (CAC) of micelles was measured by fluorimetry and results indicated low CAC value of micelles (1.9 × 10-3 g/L). ATRA was encapsulated in the core of micelles using different ratios of drug to copolymer. In the case of 10% drug to polymer ratio, more than 80% of the drug was released within 3 days, whereas for ratio of 2% more than 90% of the drug was released within 3 h. The cytotoxic study performed by MTT assay showed that H1299 survival percent decreased significantly (P ≤ 0.05) after exposure to drug-loaded micelles, while no proliferation inhibition effect was observed by either free ATRA or blank PCL-PEG-PCL micelles
Keywords:
Poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) , Micelle , All trans retinoic acid (ATRA) , H1299 , Cytotoxicity
Authors
Saeedeh Khazaei
Department of Pharmaceutical Biomaterials, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran