The combination of Coffee and tamoxifen is effective against breast cancer

Introduction & Aim: Breast cancer is the biggest health threat to women. Tamoxifen is a non-steroidal anti-estrogen that is widely used to treat breast cancer. Combination therapies are one of the therapeutic strategies that can help enhance the performance, reduce the dose, and shorten the consumption period of tamoxifen. This study aimed to investigate the synergistic effect of tamoxifen and coffee on the MCF-7 into BALB/c mice. Methods: Female Balb/c mice (5-7 weeks old) were used in this study. Tumors were established using MCF-7tumor transplantation method. Treatment began after two weeks, when tumors reached an appropriate size, the first, second, and third groups of BALB/c mice were injected with 17beta-estradiol and tamoxifen dissolved in dimethyl sulfoxide (DMSO). coffee and tamoxifen at doses at doses of 2, 5, 10, and 20 μM, of 10, 50,100 and 200 μM, and PBS over a 14-day period respectively. Then, the tumor volume was measured using a caliper. TUNEL assay kit was used to detect apoptosis. Results: Data were analyzed using the one-way analysis of variance (ANOVA). The results showed a significant difference between the negative control group and tamoxifen-treated group in terms of the simultaneous injection of coffee extract and tamoxifen (P<0.05). The results also showed increased apoptosis in this synergy.Conclusion: Tamoxifen prevents estrogen from binding to cancer cells, thus stopping cancer cell growth. This study aimed to investigate the synergistic effect of tamoxifen and coffee. The results of this study are consistent with this finding and apoptosis has increased.

Keywords:

Tamoxifen , coffee , Breast Cancer , hormonotherapy