Cardiac ECM Hydrogel Coating with A Compound Cocktail Induce Hadscs to Cardiomyocyte Like Cells

Background: The researches demonstrated that inductive fac-tors alone were not sufficient for the differentiation of stem cells into cardiomyocytes. Recently, cardiac extracellular ma-trix (cECM) coating provided tissue specific microenvironment and act as inductive template for efficient cell differentiation. The aim of this study was to investigate the effect of cardiac ECM (cECM) on cardiomyocyte-like cell differentiation of hu-man adipose tissue-derived stem cells (hADSCs) using cECM hydrogel in combination with a cardiac inductive cocktail.Materials and Methods: After approval of characteristics of hADSCs and cECM hydrogel, hADSCs culture on coated ECM with and without inductive cocktail for 3weeks. qRT-PCR and western blot were used for evaluating the expression pattern of special cardiac genes and proteins.Results: Our study showed enhanced expression of GATA4, HAND1, HAND2, NKX2.5, Troponin I, βMHC, Connexin43 genes, and Connexin43, cTnI and βMHC proteins.Conclusion: We confirmed that cECM by itself could affect vi-ability, proliferation and differentiation of hADSCs. However, using cECM in combination with a cardiac inducing cocktail improved the results. Also hADSCs can be considered as an appropriate source for cardiac tissue engineering.

Keywords:

Cardiac Extracellular Matrix , Tissue Engineering , Cardiomyocyte-Like Cell , Human Adipose Tissue-Derived Stem Cells , Differentiation