Cytotoxicity Effect of Trans Cinnamic Acid on Hela Cancer Cell Line

Background: HeLa is the most widely used model cell line for studying human cellular and molecular biology. Cervical cancer is the third most common cancer and the fourth cause of death due to cancer among women in the world, accounting for more than 80% of deaths from this cancer type in develop-ing countries. Polyphenolic compounds were used as natural anticancer agents due to anti-inflammatory, anti-oxidant, anti-mutagenic and inhibitory effects and tumor growth. The aim of this study is to investigate the effect of trans cinnamic acid on Hela cancer cell line.Materials and Methods: Hela cells were seeded and treated with 5, 25, 50 and 100 mM concentrations of trans cinnamic acid for 1, 3 and 5 days. Cell viability was evaluated on certain days after treatment. We used MTT assay to confirm cell viabil-ity. The absorbance of plates was measured at 570 nm using an ELISA reader. Also, Acridin Orange (AO) staining was used to detect apoptosis.Results: We observed a significant change in cell viability of treated cells with tran cinnamic acid. AO staining confirmed apoptotic morphology in treated cells.onclusion: Therefore, we conclude that trans cinnamic acid had an anticancer effect on Hela cells.

Keywords:

HeLa , Acridin Orange , Trans Cinnamic Acid , MTT Assay