Antibacterial effect of Alcoholic extract of Ruscus Hyracanus on Staphylococcus aureus

Introduction: Staphylococcus aureus is major pathogens in humans. The most common food poisoning is caused by Staphylococcus aureus. Misuse of antibiotics, leads to the emergence of antibiotic-resistant pathogens and resulting in greater need for new drugs or more effective medications. Ruscus hyracanus has medicinal properties and has antioxidant, anti-inflammatory, antimicrobial and anticancer effects. The aim of this study was to investigate the antibacterial effect of alcoholic extract of Ruscus hyracanus on S. aureus bacteria. Materials and Methods: The plant sample was collected from the forests of the city of Amol and was removed from direct sunlight in a shadow then the dried sample by the mill was powdered and stored. Extraction was done by maceration (soaking). To evaluate the antimicrobial effect by microdilution, concentrations of 2.5, 5, 10, 20, 40 and 80 mg/ml of extract were used. MIC (Minimum Inhibitory Concentration) and MBC (Minimum Concentration Rate) of extracts were determined. Results: S. aureus was almost susceptible to concentrations of 10, 20, 40 and 80 mg/ml of ethanolic extract of Ruscus hyracanus. Staphylococcus aureus inhibited at MIC 5mg/ml of ethanolic extract and MBC 10 mg/ml. Conclusion: According to the results, the Ruscus hyracanus had antibacterial activity agains S. aureus. This study could be a better and more accurate understanding of medicinal plants traditionally used without limitations for many years as a safe drug and without anxiety about side-effects.

Keywords:

Antimicrobial activity , Ruscus Hyracanus , Staphylococcus aureus