Green synthesis and antibacterial effect of gold nanoparticles using Crataegus microphylla extract

Background and Objective: Nanotechnology is the most progressive area of research in the present era. There is a growing interest in the green synthesis of nanoparticles using plant extract because the technique is cost effective, eco-friendly and environmentally benign. The synthesis and biomedical application of noble metal nanoparticles an especially gold nanoparticle is a promising field of research and has garnered the attention of vast groups of researchers. Bulk metallic gold (Au) is known to be chemically inert. However, when the size of gold is reduced to nanometer ranges it starts to show diverse biological properties. Materials and Methods: A rapid one step green synthetic method using Crataegus microphylla extract as a reducing agent was employed for preparation of gold nanoparticles. The synthesized gold nanoparticles had been characterized by Fourier transform infrared spectroscopy (FT-IR), X-Ray diffraction (XRD), scanning electron microscopy and energy-dispersive X-ray spectroscopy (EDX) techniques. Also the synthesized gold nanoparticles were investigated for their antibacterial activity (Staphylococcus aureus, Acinetobacter, klebsiella and Enterococcus) and MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) were recognized. Findings: The results indicated that the synthesized gold nanoparticles were crystalline, stable, well dispersed and spherical in morphology and had an average diameter of 30-40 nm. The prepared nanoparticles were found to be biocompatible and exhibited no antibacterial effect, deeming the particles safe for various applications in nanomedicine. Conclusion: The aim of this study was green synthesis of gold nanoparticles through the co-precipitation rout and in presence of Crataegus microphylla extract. The Crataegus microphylla extract capped gold nanoparticles are highly stable and have showed antibacterial activity on both gram-positive and gram-negative bacteria.

Keywords:

Gold Nanoparticles , Green synthesis , Antibacterial.