Microscopic characterization, TLC fingerprinting and determination of total phenol and flavonoid of different population of Camellia sinensis (L.) Kuntze (green tea) compared to a standard sample
Publish place: Trends in Pharmaceutical Sciences، Vol: 5، Issue: 2
Publish Year: 1398
نوع سند: مقاله ژورنالی
زبان: English
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تاریخ نمایه سازی: 8 دی 1398
Abstract:
With respect to the importance and high consumption of this drink, assessment of various brands and populations are needed to reach to high quality samples. Low-quality or fake samples may cause many unwanted effects. Therefore, comparative study of various samples will be beneficial to select a high quality sample. This study aimed to assess evaluate the quality of fourteen green tea samples based on microscopic characterization and pharmacognostical properties. Fourteen green tea samples were purchased from markets (Iran), both internal and external brands. Also a sample was collected from Lahijan (North of Iran) as a control. The methanol extracts of all samples were subjected to microscopic characterization as well as determination of total phenol and flavonoid content using current related methods. In addition, thin layer chromatography (TLC) fingerprint was performed on all respected extracts using HPTLC technique. Microscopic characterization showed calcium oxalate crystal, trichomes, idioblasts, stroma in high quality samples. Total phenol content of Chinese teabag methanol extract was at highest (288.4±12.03 mg GAE/g extract) and the lowest phenol content was related to the Chinese bulk sample (144.76±4.32 mg GAE/g extract). On the other hand, highest and lowest flavonoid content was found in Pakistan (19.77±0.68 mg QE/ g extract) and Lahijan (4.5±0.02 mg QE/ g extract) bulk samples, respectively. TLC chromatogram fingerprint indicated the presence of phenolic compounds with related intensity. Current study represented that these assessments are well functional and beneficial to be consider for the screening and quality control evaluation of various tea samples from different origins.
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Authors
Zahra Shahbazi
Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Mohammad M. Zarshenas
Department of phytopharmaceuticals (Traditional Pharmacy), School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran. |Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Mahmoudreza Moein
Department of Pharmacognosy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.
Sedigheh Khademian
Department of Phytopharmaceuticals (Traditional Pharmacy), School of Pharmacy, Shiraz University of Medical Science, Shiraz, Iran