Investigate the effect of hydroalcoholic dill extract on plasma levels of hypothalamic-pituitary-gonadal axis hormones in male mice

Background and Aim : The hypothalamic-pituitary-gonadal axis is one of the important neuroendocrine pathways. Dill contains various chemical compounds such as flavonoids and flavonoids,that they have estrogenic properties. These compounds have many effects on the hypothalamic-pituitary-gonadal axis. According to side effects of chemical drugs affecting the hypothalamus-pituitary-gonadal axis, finding plants with similar properties will be useful. The aim of this study was to determine the effect of dill extract on blood concentrations of gonadotropic and testosterone hormones in male mice.Methods : In this study, 28 male mice were divided into 4 groups: control, dill 100, dill 300 and dill 500 mg / kg. Hydro-alcoholic extract of dill was used for 30 days by intraperitoneal injection. After this period (30 days) under complete anesthesia, blood was collected from the hearts of the animals. Serum blood samples were measured by using specific kits of hormone radioimmunoassay.Results : The results of the present study showed that the serum level of testosterone in the extract receiving groups was significantly lower than the control group. But the levels of LH and FSH in the groups receiving the extract increased in a dose-dependent manner.Conclusion : Increased pituitary-secreting hormones appear to be due to a decrease in testosterone secreted by the testes and this decrease activates the negative feedback in the hypothalamic-pituitary-gonadal axis. As a result, it increased the LH and FSH hormones in the extract receiving groups.