The effect of Cinnamomum Zeylanicum extract pre-nutrition on prevention of stroke in high-fat diet receiving rats

Background and Aim : Acute ischemic stroke is the most frequent cause of permanent disability in adults worldwide and the second leading cause of death after cancer and heart disease in Iran. According to the previous studies, the anti-inflammatory, anti-oxidant, anti-blood lipid and anti-angiogenesis properties of cinnamon have been proven. In this study, the pre-nutritional effect of hydroalcoholic extract of cinnamon on the expression of genes involved in the destruction of the blood-brain barrier and its increased permeability after cerebral stroke was studied after receiving a high-fat diet.Methods : In this study,60 Wistar rats were divided into 5 groups (n=12): control, sham, model, low dose cinnamon (130 mg/kg) and high dose cinnamon (260 mg/kg). All groups except the control group were on a high-fat diet for 8 weeks. Stroke was induced through Middle Cerebral Brain Artery Occlusion (MCAO). 12 hours after the induction of stroke, the animals were sacrificed and brain tissue evaluated for the expression of calpainI, calpainII, occludin, and VEGF genes and P-selectin protein.Results : Hydroalcoholic extract of high-dose cinnamon has been effective in reducing the expression genes of calpainII, vegf, and P-selectin proteins and increasing the expression of occludin gene (P<0/001). Low dose and high dose cinnamon decreased significantly the expression of calpainI gene (P<0/001).Conclusion : Pre-treatment with Hydroalcoholic Extract of Cinnamon reduced the calpainI, calpainII,vegf mRNA levels and level of P-selectin protein and increase occludin mRNA level. thereby prevent the permeability of the blood-brain barrier and its subsequent edema in ischemia-reperfusion.

Keywords:

Stroke , calpainI , calpainII , occludin , P-Selectin , vegf