Optimization of Trichoderma reesei Culture Environment Using Nitrogen Organic References to Increase Xylanase Enzyme Production

Introduction and purpose:Trichoderma is one of ascomycete mesophile fungi, which exist in all soils and are part of the majority of soil’s cultivable fungi. Xylanases are part of hydrolases and possess single chains of glycoproteins whose weighs range from 6 to 8 KD. Xylanase has multiple applications in various industries. In this study, we try to optimize Trichoderma reesei medium in order to increase Xylanase enzyme Production.Methods:We provided the standard Trichoderma reesei strain from the microbe collection of Iranian Research Organization for Science and Technology (IROST). Then, we measured the activity of its Xylanase enzyme using the standard method of DNSA. Next, for the sake of optimization, different parameters of the medium like carbon, nitrogen, and pH sources, temperature and incubation timespan were examined. The activity of the enzyme was measured at each stage using DNSA method.Results:The parental strain of Trichoderma reesei is featured by the xylanase enzyme activity of 2/59 U. the strain at the present of wheat bran, ammonium nitrate, yeast extract respectively as the carbon, inorganic nitrogen, organic nitrogen, and at 7/5 pH and with the average of 35 °C had the most amount of production.Conclusion:Xylanase enzyme is one of the most important enzymes used in diverse industries but unfortunately is not produced in our country. As it can be seen from the results of this study,we can optimize the conditions for cultivating different organisms so as to increase the industrial production of various enzymes which are not produced in Iran. We hope, thereby, to make such a production easier at the industrial scale.

Optimization of Trichoderma reesei Culture Environment Using Nitrogen Organic References to Increase Xylanase Enzyme Production Keywords:

Trichoderma reesei , Xylanase , Organic Nitrogen source.