Preparation and biodistribution study of 67Ga-gallium calcitonin
Publish place: Iranian Journal of Nuclear Medicine، Vol: 19، Issue: 2
Publish Year: 1390
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IRJNM-19-2_005
تاریخ نمایه سازی: 21 بهمن 1398
Abstract:
Introduction: In order to develop a radiolabeled calcitonin (CT) derivative for receptor imaging studies, CT was successively labeled with 67Ga-gallium chloride. Methods: The best results of the conjugation were obtained by the addition of 0.5 ml of a CT nasal pharmaceutical solution (1100 IU) to a glass tube pre-coated with DTPA-dianhydride (0.01 mg) at 25°C with continuous mild stirring for 30 min. Results: After solid phase purification of the radiolabeled hormone, instant thin layer chromatography (ITLC) showed radiochemical purity of higher than 95 % at optimized conditions (specific activity =67-134 KBq/IU, labeling efficiency 70%). 67Ga-DTPA-CT mainly accumulates in the liver. Conclusion: Preliminary in vivo studies (ID/g%) in male wild-type rats showed significant liver uptake of the tracer after 24 hours. 67Ga-DTPA-CT can be a suitable probe for biodistribution study of CT receptors in various physiological as well as neoplastic lesions with over-expressed calcitonin receptors.
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Authors
Amir Reza Jalilian
Radiopharmaceutical Research and Development Lab, Nuclear Science and Technology Research Institute, Atomic Energy Organization of Iran, Tehran, Iran
Mohammad Amin Hosseini
Radiopharmaceutical Research and Development Lab, Nuclear Science and Technology Research Institute, Atomic Energy Organization of Iran, Tehran, Iran
Fatemeh Bolourinovin
Radiopharmaceutical Research and Development Lab, Nuclear Science and Technology Research Institute, Atomic Energy Organization of Iran, Tehran, Iran