Prevalence of Resistant plasmids and integron Classes in new emerged Staphylococcus lugdunensis infection in burn patients

Introduction and objectives: Staphylococcus lugdunensis (SL) is coagulase-negative staphylococci may cause various infections with unusual severity. In resentment of various antibiotics treatment, infections caused by these bacteria are still significant and the frequency of antibiotic resistance genes, especially by Integron structures and plasmid gene increases this issue. The object of this study was to investigate the antibiotic susceptibility pattern, the presence of integron classes and presence of antibiotic resistance plasmids gene in SL isolates. Materials and Methods: Sampling for this study was carried out over a period of one year from burn patients. Twenty-eight isolates of SL were confirmed by phenotypic and Antibacterial Resistance pattern was determined by disk diffusion test (CLSI 2018). The prevalence of antibiotic resistance genes (blaZ, dfrA, ermB, ermC, ileS, mphBM and msrA) on plasmids and Integron classes’ detection were investigated by PCR method. Results: The frequency of SL was about 21.8% in burn patients of recent study. The presence of resistance genes was as follows: dfrA 28(100%), mphBM 27(96.4%), msrA 25(89.3%), blaZ 24(85.7%), ermC 24(85.7%), ermB 15 (53.6%), ileS 8(28.6%). The prevalence of integron classes I and II was 7 (25.00%) and 2 (7.14%) respectively while class III integron was detected. The association of antibiotic resistance and the presence of integron classes was not significant. A significant relation between resistance genes and antibiotic resistant was seen. Conclusion: The results indicated the probability of association between antibiotic resistance pattern and presence of resistance plasmid genes. Presence of genes on plasmids increase the probable of resistant transferring in nosocomial infections. Therefore, checking of resistant transfer mechanisms and high risks is helpful in controlling the hospital infections caused by this bacterium.

Keywords:

Staphylococcus lugdunensis , Integrons , Shiraz , Antibiotic resistance , plasmid