Publisher of Iranian Journals and Conference Proceedings

Please waite ..
CIVILICAWe Respect the Science
Publisher of Iranian Journals and Conference Proceedings
عنوان
Paper

Molecular detection of AdeFG efflux pump genes in clinical isolates of Acinetobacter baumannii and their role in antibiotic resistance in Tehran

تعداد صفحات: 1 | تعداد نمایش خلاصه: 46 | نظرات: 0
سال انتشار: 1398
کد COI Paper: MEDISM20_344
زبان Paper: Englishglish
نسخه کامل Paper در کنفرانس ارائه نشده است و در دسترس نیست.

مشخصات نویسندگان Paper Molecular detection of AdeFG efflux pump genes in clinical isolates of Acinetobacter baumannii and their role in antibiotic resistance in Tehran

Mohammad Niakan - Department of Medical Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran.
Reza Mirnejad - Molecular Biology Research Center, System biology and Poisoning institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
Rasoul Kaviani - Department of Medical Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran.
Iman Pouladi - Department of Medical Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran.
Zahra salahi - Department of Medical Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran.

چکیده Paper:

Introduction and Objectives: Acinetobacter baumannii is one of the most important causes of hospital bacterial infections in the world. recently antibiotic-resistant strains of A. baumannii have proliferated, which may be due to the efflux pumps. Materials and Methods: In this study, 200 clinical Isolates from ulcer, pus, sputum, and blood were collected in Mostafa Khomeini, Tohid and Motahari hospitals in Tehran, and their identity was verified by conventional standard biochemical tests. Then, the antibiotic-sensitivity pattern was determined by the disc diffusion method in the presence and absence of efflux pump inhibitors in samples according to the CLSI instructions. AdeFG efflux pump genes in samples were identified by PCR method. Results: In this study, 60 isolates of A.baumannii bacteria were identified based on biochemical differential tests. The identity of all samples was verified by PCR and blaOXA-51-like gene amplification methods. Investigation of the antibiotic-resistance of samples showed that 98.34% of the samples were resistant to the three antibiotics of ciprofloxacin, norfloxacin and levofloxacin. According to results the of PCR method, 100% of the 60 A. baumannii samples involved the AdeF gene and 76.66% involved the AdeG gene. Conclusion: The results of this study indicate that resistance to all three antibiotics of ciprofloxacin, norfloxacin and levofloxacin exists in AdelaFG gene-carrying strains due to the efflux pump and they have a significant relationship. The role of other factors and mechanisms involved in inducing the resistance should not be ignored.

کلیدواژه ها:

Acinetobacter baumannii, efflux pump, antibiotic resistance, AdeFG

کد Paper/لینک ثابت به این Paper

برای لینک دهی به این Paper می توانید از لینک زیر استفاده نمایید. این لینک همیشه ثابت است و به عنوان سند ثبت Paper در مرجع سیویلیکا مورد استفاده قرار میگیرد:

https://civilica.com/doc/987460/

کد COI Paper: MEDISM20_344

نحوه استناد به Paper:

در صورتی که می خواهید در اثر پژوهشی خود به این Paper ارجاع دهید، به سادگی می توانید از عبارت زیر در بخش منابع و مراجع استفاده نمایید:
undefined, undefined و undefined, undefined و undefined, undefined و undefined, undefined و undefined, undefined,1398,Molecular detection of AdeFG efflux pump genes in clinical isolates of Acinetobacter baumannii and their role in antibiotic resistance in Tehran,بیستمین کنگره بین المللی میکروب شناسی ایران,Kerman,,,https://civilica.com/doc/987460

در داخل متن نیز هر جا که به عبارت و یا دستاوردی از این Paper اشاره شود پس از ذکر مطلب، در داخل پارانتز، مشخصات زیر نوشته می شود.
برای بار اول: (1398, Niakan, Mohammad؛ Reza Mirnejad و Rasoul Kaviani و Iman Pouladi و Zahra salahi)
برای بار دوم به بعد: (1398, Niakan؛ Mirnejad و Kaviani و Pouladi و salahi)
برای آشنایی کامل با نحوه مرجع نویسی لطفا بخش راهنمای سیویلیکا (مرجع دهی) را ملاحظه نمایید.

Research Info Management

Certificate | Report Paper

Export Citation info of this Paper to research management softwares

علم سنجی و رتبه بندی Paper

مشخصات مرکز تولید کننده این Paper به صورت زیر است:
نوع مرکز: state university
تعداد مقالات: 3,915
در بخش علم سنجی پایگاه سیویلیکا می توانید رتبه بندی علمی مراکز دانشگاهی و پژوهشی کشور را بر اساس آمار مقالات نمایه شده مشاهده نمایید.

New RelatedPapers

Share this paper

WHAT IS COI?

COI is a national code dedicated to all Iranian Conference and Journal Papers. the COI of each paper can be verified online.

Support