Year: 1398
Publish place: Disease and Diagnosis، Vol: 8، Issue: 1
COI: JR_IEJM-8-1_005
Language: EnglishView: 229
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Abstract:
Background: Regarding the emergence and increasing of multidrug-resistant strains among Klebsiella pneumoniae nosocomial isolates the therapeutic options for the treatment has been limited. The β-lactamases enzymes are the major defense of gram-negative bacteria against antibiotics. The aim of this study was to the detection ofblaCTX-M, blaSHV , and blaTEM genes among K. pneumoniae strains isolated from patients who admitted to Shahid Beheshti hospital of Babol city, Iran, in 2016. Materials and Methods: This study was conducted on 50 samples of K. pneumoniae strains isolated from hospitalized patients. Antibiotic susceptibility testing was performed by Kirby- Bauer disc diffusion method according to CLSI guidelines. The blaCTX-M, blaSHV, and blaTEM genes were detected by polymerase chain reaction method. Result: Among studied strains, the prevalence of blaCTX-M, blaSHV , and blaTEM genes were 24 (49%), 44 (88%), and 36 (72%), respectively. In this study, imipenem and nitrofurantoin were more effective than other antibiotics. Also, 100% of strains were susceptible to imipenem. Conclusions: The prevalence of antibiotic resistance genes detected in this study implies a great concern for the treatment of multidrug-resistant K. pneumonie. Hence, infection control measures, including antibacterial management and identification of resistant isolates for preventing of nosocomial outbreaks have become highlighted
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