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In vitro cytotoxicity of folate-silicagold nanorods on mouse acute lymphoblastic leukemia and spermatogonial cells

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Year: 2018
COI code: ISERB04_076
Paper Language: English

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Authors In vitro cytotoxicity of folate-silicagold nanorods on mouse acute lymphoblastic leukemia and spermatogonial cells

  Neda Eslahi, - M.SC Department of Anatomical Sciences, School of Medicine,Iran University of Medical Sciences, Tehran, Iran
  ALI Shakeri-Zadeh - Department of Medical Physics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  Khadijeh Ashtari, - PHD Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. -Department of Medical Nanotechnology and Faculty of advanced technology in Medicine, Iran University of Medical Sciences, Tehran, Iran
  Vahid Pirhajati-Mahabadi, - PHD,Neuroscience Research Center, Iran University of Medical Sciences, Tehran, Iran

Abstract:

Background: The purpose of this study was to evaluate in vitro cytotoxicity of GNRs on the viability of spermatogonial cells and mouse acute lymphoblastic leukemia cells (EL4s). Methods: Spermatogonial cells were isolated from the neonate mice, following enzymatic digestion and differential plating. GNRs were synthesized, then modified by silica and finally conjugated with folic acid to form F-Si-GNRs. Different doses of F-Si-GNRs ( 25, 50, 75, 100, 125 and 140 μM) were used on the both cells. MTT assay was performed to examine the GNRs toxicity. Flow cytometry was used to confirm the identity of the EL4s and spermatogonial cells. Also, the presence of spermatogonial cells asdetermined by the expression of specific spermatogonial genes and transplantation into recipient testes. Apoptosis was determined by flow cytometry using an annexin V/propidium iodide kit. Results: Flow cytometry showed that SSCs and EL4s were respectively PLZF and H-2kb positive. The percentage viability of SSCs and EL4s that were treated with 25, 50, 75, 100, 125 and 140 μM of F-Si-GNRs was 65.33±3.51%, 60±3.6%, 51.33±3.51%, 49±3%, 30.66±2.08% and 16.33±2.51% for SSCs and 57.66±0.57%,54.66±1.5%, 39.66±1.52%, 12.33±2.51%, 10±1% and 5.66±1.15% for EL4s respectively. The results of the MTT assay indicated that 100 μM is the optimal dose to reach the highest and lowest level of cell death in EL4s and in spermatogonial cells, respectively. Conclusion: As conclusion, cell death increased alongside increasing concentrations of F-Si- GNRs. Following utilization of F-Si-GNRs, there was a significant difference in the extent of apoptosis between cancer cells and SSCs.

Keywords:

Spermatogonial cells; Gold Nanorods;Mouse acute lymphoblastic leukemia cells (EL4s); Folic acid; Cytotoxicity

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COI code: ISERB04_076

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Eslahi,, Neda; ALI Shakeri-Zadeh; Khadijeh Ashtari, & Vahid Pirhajati-Mahabadi,, 2018, In vitro cytotoxicity of folate-silicagold nanorods on mouse acute lymphoblastic leukemia and spermatogonial cells, Fourth International Congress on Reproduction, تهران, انجمن علمي جنين شناسي و بيولوژي توليد مثل ايران, https://www.civilica.com/Paper-ISERB04-ISERB04_076.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Eslahi,, Neda; ALI Shakeri-Zadeh; Khadijeh Ashtari, & Vahid Pirhajati-Mahabadi,, 2018)
Second and more: (Eslahi,; Shakeri-Zadeh; Ashtari, & Pirhajati-Mahabadi,, 2018)
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Type: Medical University
Paper No.: 3023
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