Determination of genetic uniformity in transgenic cotton plants usingDNA markers (RAPD and ISSR) and SDS-PAGE

One concern about using transgenic plants is the Un-uniformity that derived from somaclonal variationmolecular markers play an important role in the efficient and effective determination of genetic variation. The presentwork was carried out to assess the genetic uniformity of transgenic cottons (Bt and chitinase lines), using RAPD, ISSRmolecular markers and SDS-PAGE analysis. Similarity matrix for RAPD marker based on Nei and Li’s coefficientrevealed that p-values for pairwise comparisons between Bt and chitinase cottons and their conventional counterpart plantwere 93% and 95% respectively. Also, similarity matrix for ISSR marker revealed that p-values for pairwise comparisonsbetween Bt and chitinase lines and their conventional counterpart were 94% and 96% respectively. Pairwise similaritycoefficient among two transgenic plants and their conventional counterparts ranged from 0.94 to 0.99 SDS-proteinpatterns exhibiting four major bands. Also, molecular weight ranged from 10 KD to 35 KD. Similarity matrix for proteinpatterns revealed that pairwise value between Bt and chitinase lines and their conventional counterpart was 100%. Allprotein bands of three cultivars were the same and no polymorphic bands were detected. Pairwise similarity coefficientamong two transgenic plants and their conventional counterpart ranged from 0.94 to 0.99. These lines could be used formore in-depth evaluation and new ways of commercializing biosafety studies can be proposed.

Keywords:

Genetic uniformity , ISSR , RAPD , SDS-PAGE , Transgenic cotton plants