Preparation and characterization of a dry powder inhalation (DPI) containing solid nanoparticles encapsulating amphotericin B
Publish place: First National Congress on Nanotechnology in Health Sciences
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
NHSMED01_030
تاریخ نمایه سازی: 18 آذر 1398
Abstract:
Background and Objective:The aim of this study was to prepare dry powder inhalers (DPIs) containing amphotericin B solid lipid nanoparticles (AMB-SLNs).Preparation of DPI formulations containing solid lipid nanoparticles encapsulating AMB-DSPG complex was studied as an alternative way for prevention of pulmonary aspergillosis.Materials and Methods:For improving the solubility of amphotericin B in organic solvents, ion paired complexes were formed by establishing electrostatic interaction between amphotericin B and distearoyl phosphatidylglycerol (DSPG). The SLN formulations containing AMB-DSPG complexes were prepared using glycerol monostearate (GMS) as the lipid matrix and soybean lecithin and tween 80 as the surfactant by solvent emulsification-evaporation technique. The nanoparticles were optimized through a fractional factorial design. DPIs were prepared by lyophilization technique using lactose as the inhalational carrier and then after, the formulations were evaluated in terms of aerodynamic particle size distribution using a cascade impactor. The morphology of the particles was examined using scanning electron microscopy. The in vitro drug release profiles were evaluated.Findings:The particle size, PdI, zeta potential, entrapment efficiency and drug loading capacity of the optimized SLNs were 187.04±11.97 nm, 0.188±0.028, -30.16±1.6 mV, 89.3±3.47 % and 2.76±0.32 %, respectively. Formulation containing 10%w/v of lactose with the calculated fine particle fraction value as 72.57±4.33 was exhibited the appropriate aerodynamic characteristics for pulmonary drug delivery. SEM images revealed de-agglomerated particles. In vitro release studies showed sustained release of AMB and the release kinetics were best fitted to the first order kinetic model.Conclusions:The results found here indicated that AMB-SLNs could be successfully prepared and stabilized through freeze drying.
Keywords:
Amphotericin B , Ion paired complexation , distearoyl phosphatidylglycerol (DSPG) , Solid lipid nanoparticles (SLN) , Dry Powder Inhalation (DPI)
Authors
Reza Mahjub
Department of Pharmaceutics, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran
Ehsan Mehrabani Yeganeh
Department of Pharmaceutics, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran
Hossein Bagheri
Department of Pharmaceutics, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran