Morphological and molecular identification and pathogenecity test of Colletotrichum spp. of citrus in Iran
صاحب اثر: سازمان تحقیقات، آموزش و ترویج کشاورزی
نوع محتوی: طرح پژوهشی
Language: Persian
استان موضوع گزارش: مازندران
شهر موضوع گزارش: رامسر
Document National Code: R-1051312
Publish: 16 February 2019
دسته بندی علمی: علوم کشاورزی
View: 504
Pages: 52
Publish Year: 1395
نسخه کامل Research منتشر نشده است و در دسترس نیست.
Morphological and molecular identification and pathogenecity test of Colletotrichum spp. of citrus in Iran abstract
Colletotrichum species are causal agents of anthracnose, postbloom fruit drop, tear stain and postharvest fruit rot in citrus. In recent years, the damage of these diseases has increased in citrus orchards, especially in the North of Iran. In the last few years there have been many changes in classification of this genus, so, to study the causal agents of disease, mrphological, phylogenetical and pathogenecity studies of Colletotrichum species associated with citrus trees in Iran and introducing the common species were studied.To identify Colletotrichum species, sampling from citrus orchards in Golestan, Mazandaran, Guilan, Fars, Hormozgan and Kerman provinces were done. In this study 292 isolates were obtained from leaves, fruits, stems and flowers of Citrus species and their morphological characters such as, conidium shape and size, appressorium shape, color of colony and presence or absence of setae were studied. Due to huge number of isolates, some of them were selected for phylogeny study and pathogenecity test. In multilocus phylogenetic analysis a part of TUB2, CHS-1 and CAL genes of 13 selected isolates were ampelified, sequenced and compared by BLAST search with related sequences retrieved from GenBank and their phylogenetic trees were constructed. Based on morphological and molecular data, five species including C.gloeosporioides s.str., C. fructicola and C. siamense (from C.gloeosporioides complex); C. karstii and C. novae zelandiae (fromC.boninense complex) were identified. All species, except C. novae-zelandiae were pathogenic on citrus.Keywords: Morphology, Pathogen, Phylogeny, Species complex,
Morphological and molecular identification and pathogenecity test of Colletotrichum spp. of citrus in Iran authors