Effect of Glaucium Flavum Extract on Bax and Bcl2 Genes Expression in HT-29 Cancer Cells

Introduction and Objectives: Cancer is still considered an issue in society; each year, 2.8 people die from this condition; therefore, the discovery or design of antineoplastic agents with little or no toxicity is necessary. Medicinal herbs are widely used today to treat cancer. The present study aimed at investigating the effect of Glaucium flavum extract on apoptosis induction in HT-29 cell line.Material and Methods: Different concentrations of Glaucium flavum extract (25, 50, 100, and 200 μg/ml) were added to HT-29 cells in the MTT assay. The metabolic activities of the treated cells were studied for 5 hours according to the ODs read by the Elisa-reader. QRT-PCR technique was used to evaluate the expression of Bax, Bcl2 gene. The statistical analysis carried out using SPSS software and One-way-ANOVA test.Results: Analysis of QRT-PCR data showed that the concentration of 50 (μg/ml) as IC50 during 4-hour treatment could increase Bax gene expression and decrease expression of Bcl2.Conclusion: This extract induces apoptosis and cell death as a result of the effects of its alkaloidal compounds.

Keywords:

Glaucium flavum , Beclin1 , HT-29 , Autophagy