Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium

Publish Year: 1399
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_EHEM-7-2_007

تاریخ نمایه سازی: 16 دی 1399

Abstract:

Background: Biodegradation of hydrocarbon compounds is a great environmental concern due to their toxic nature and ubiquitous occurrence. In this study, biodegradation potential of oily soils was investigated in an oil field using indigenous bacterial consortium. Methods: The bacterial strains present in the contaminated and non-contaminated soils were identified via DNA extraction using 16S rDNA gene sequencing during six months. Furthermore, total petroleum hydrocarbons (TPH) were removed from oil-contaminated soils. The TPH values were determined using a gas chromatograph equipped with a flame ionization detector (GC-FID). Results: The bacterial consortium identified in oil-contaminated soils (case) belonged to the families Halomonadaceae (91.5%) and Bacillaceae (8.5%), which was significantly different from those identified in non-contaminated soils (control) belonging to the families Enterobacteriaceae (84.6%), Paenibacillaceae (6%), and Bacillaceae (9.4%). It was revealed that the diversity of bacterial strains was less in oil-contaminated soils and varied significantly between case and control samples. Indigenous bacterial consortium was used in oil-contaminated soils without need for amplification of heterogeneous bacteria and the results showed that the identified bacterial strains could be introduced as a sufficient consortium for biodegradation of oil-contaminated soils with similar texture, which is one of the innovative aspects of this research. Conclusion: An oil-contaminated soil sample with TPH concentration of 1640 mg/kg was subjected to bioremediation during 6 months using indigenous bacterial consortium and a TPH removal efficiency of 28.1% was obtained.

Authors

Yalda Basim

Environmental Technologies Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Ghasemali Mohebali

Microbiology and Biotechnology Research Group, Research Institute of Petroleum Industry, Tehran, Iran

Sahand Jorfi

Environmental Technologies Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Ramin Nabizadeh

Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Mehdi Ahmadi Moghadam

Environmental Technologies Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Ata Ghadiri

Department of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Nematollah Jaafarzadeh Haghighi Fard

Corresponding author:Environmental Technologies Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran