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Title

Investigation of transgenic elements in genetically modified maize germ (Zea mays) and maize germ oil distributed in local market by qualitative PCR method

Year: 1399
COI: JR_JHHHM-1-2_009
Language: EnglishView: 54
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Authors

Hossein Rastegar - Islamic Azad University, Medical Sciences Branch, Faculty of pharmacy, Tehran, Iran.
Roya Khosrokhavar - Food and Drug Laboratory Research Center, Iran Food and Drug Administration, Ministry of Health and Medical Education, Tehran, Iran.
Shahram Shoeibi - Food and Drug Laboratory Research Center, Iran Food and Drug Administration, Ministry of Health and Medical Education, Tehran, Iran.
Mehdi Jafariasl - Zar Grain Refinery, Zarnam Farhikhtegan Industrial Research Group, Tehran, Iran.
Arash Ershadi - Zar Grain Refinery, Zarnam Farhikhtegan Industrial Research Group, Tehran, Iran.
Mahdi Taherian - General Directorate of Natural, Traditional and Complementary Products, Iran Food and Drug Administration, Ministry of Health and Medical Education, Tehran, Iran.

Abstract:

Background and objective: Detection of genetically modified organisms (GMOs) is a controversial issue in food control. Due to the increased production and consumption of transgenic products in the last decade, their monitoring and characterization has come into force by the regulators. At this study, we examined the transgenic elements in maize germs and their oil by PCR. Materials and methods: Following the DNA extraction from maize germ and maize oil by CTAB and Wizard Magnetic methods, respectively, PCR test was conducted to analyze the genes CaMV35S, NOS, FMV, and zein. Then, the PCR products were electrophoresed on agarose gel 2% containing ethidium bromide. To avoid false negative and false positive results, appropriate control actions were implemented during the experiments. Results and conclusion: CaMV35S, NOS, FMV, and zein genes were detected and identified in transgenic maize germ samples, but no specific band of the transgenes was found for maize oil samples, indicating the DNA degradation during the oil production and purification process. It indicated that the current processes of maize germ oil production remove the residual transgenes. Therefore, the concerns about unintended intake of unknown genetic materials through the genetically modified crops such as maize oil is reduced to some extent.

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Paper COI Code

This Paper COI Code is JR_JHHHM-1-2_009. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/1170711/

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Rastegar, Hossein and Khosrokhavar, Roya and Shoeibi, Shahram and Jafariasl, Mehdi and Ershadi, Arash and Taherian, Mahdi,1399,Investigation of transgenic elements in genetically modified maize germ (Zea mays) and maize germ oil distributed in local market by qualitative PCR method,https://civilica.com/doc/1170711

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The specifications of the publisher center of this Paper are as follows:
Type of center: Azad University
Paper count: 974
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