The Optimized Protocols for Production, Adaptation and Keeping of the Produced Artificial Seeds from Encapsulated Lateral Buds in Stevia rebaudiana (Bertoni)
Publish place: Agrotechniques in Industrial Crops، Vol: 1، Issue: 1
Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_ATIC-1-1_004
تاریخ نمایه سازی: 9 مرداد 1400
Abstract:
Stevia, as an anti-diabetic medicinal plant, is estimated to be about ۳۰۰ times sweeter than sugar cane. Low seed germination is a major problem in stevia propagation. Artificial seeds (ASs) technology is a valuable method for it’s rapid and massive propagation. In this study, the effective factors on production, the produced plantlet adaptation and keeping of the ASs produced from encapsulated lateral buds were efficiently optimized for the first time. The results showed that ۲.۵% sodium alginate with ۱% calcium chloride was the best composition for coating the ASs. The bigger lateral buds (۲-۳ mm) with MS medium resulted in the highest percent and rate of germination. Germination and growth of the ASs were increased using ۱ mg l-۱ BAP with ۱.۵ mg l-۱ Kn in the pre-culture environment. The findings indicated that transmission of the ASs-plantlets to cocopeat seedbed increased the growth characters. Keeping the produced ASs in the MS medium had a significant (p <۰.۰۵) and positive effect on germination characters. Among the treatments for keeping artificial seeds in ۹۰ days, the treatment of liquid paraffin at ۴oC showed the best response for regrowth. In this research, the possibility of routine and massive production of ASs by encapsulating the lateral buds has been reported in stevia. Therefore, using this new protocol, the rapid and fast micro-propagation of this plant could be achieved through lateral buds for inexpensive and commercial purposes in the future.
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Authors
Bahare Shaafi
Department of Agronomy and Plant, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
Sayed Saeed Mosavi
Department of Agronomy and Plant, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
Mohamad Reza Abdollahi
Department of Agronomy and Plant, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
Hasan Sarikhani
Department of Agronomy and Plant, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
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