Repetitive sequences based on genotyping of Candida albicans isolates obtained from Iranian patients with human immunodeficiency virus
Publish Year: 1393
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-17-11_002
تاریخ نمایه سازی: 4 آبان 1400
Abstract:
Objective(s): Candidiasis infection caused by Candida albicans has been known as a major problem in patients with immune disorders. The objective of this study was to genotype the C. albicans isolates obtained from oral cavity of patients with positive human immunodeficiency virus (HIV+) with or/and without oropharyngeal candidiasis (OPC). Materials and Methods:A total of ۱۰۰ C. albicans isolates from Iranian HIV+patients were genotyped using specific PCR primers of the ۲۵S rDNA and RPS genes. Results: The frequencies of genotypes A, B and C which were achieved using ۲۵S rDNA , were ۶۶, ۲۴ and ۱۰ percent, respectively. In addition, genotypes D and E were not found in this study. Each C. albicans genotype was further classified into four subtypes (types ۲, ۳, ۲/۳ and ۳/۴) by PCR amplification targeting RPS sequence. Conclusion:In general, genotype A۳ constituted the majority of understudy clinical isolates obtained from oral cavity of Iranian HIV+ patients.
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Authors
Iradj Ashrafi Tamai
Bu Ali Sina University, Hamedan, Iran Research and Training Center of Imam Khomeini Hospital, Tehran, Iran
Taghi Zahraei Salehi
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Aghil Sharifzadeh
Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Hojjatollah Shokri
Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
Ali Reza Khosravi
Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
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