Effect of Melatonin on the Expression of Apoptotic Genes in Vitrified-thawed Spermatogonia Stem Cells Type A of ۶-Day-Old Mice
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 16، Issue: 8
Publish Year: 1392
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-16-8_004
تاریخ نمایه سازی: 4 آبان 1400
Abstract:
Objective(s): Being secreted by the pineal gland, melatonin induces cell proliferation in normal cells and induced apoptosis in cancer cells. The purpose of this study was to investigate effects of melatonin on main components and the expression of apoptotic genes in vitrified-thawed testicular germ cells of ۶- day–old mice. Materials and Methods: Testes of neonate Balb/c mice were vitrified- thawed under standard condition with or without the addition of ۱۰۰ μM melatonin to both vitrification and thawing solutions. Subsequently, Vitrified-thawed wholetestes were digested under standard condition and spermatogonial stem cells type A were separate in the suspension with CD۹۰.۱ (Thy۱.۱+) micro beads. Extraction of RNA and synthesis of cDNA was performed. Expression levels of apoptotic genes (Fas, P۵۳, BCL-۲ and BAX) were determined using Real-time PCR. Results: With all genes being expressed, level of expression for Fas was higher and for that of P-۵۳ was lower than the remaining genes. Conclusion: Melatonin may cause apoptosis in cells being damaged under the influence of freezing thawing process. In order to examine the exact effects of melatonin on spermatogonia stem cells apoptosis, additional studies are required.
Keywords:
Apoptosis Melatonin Testes Vitrification
Authors
Mohammadreza Gholami
Department of Anatomy, Lorestan University of Medical Sciences, Khoramabad, Iran
Ghasem Saki
Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences (AJUMS), Ahvaz, Iran
Masoud Hemadi
Fertility, Infertility and Perinatology Research Center, Ahvaz Jundishapur University of Medical Sciences (AJUMS), Ahvaz, Iran
Ali Khodadadi
Department of Immunology, Ahvaz Jundishapur University of Medical Sciences (AJUMS), Ahvaz, Iran
Javad Mohamma di-asl
Departmentof Medical Genetics, Ahvaz Jundishapur University of Medical Sciences(AJUMS), Ahvaz, Iran
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