تعیین شرایط بهینه ی تهیه ی کمپلکس Ga-Oxine۶۷ برای نشان دارسازی سلول های سفید خون

In this work, the effective factors on the preparation of ۶۷Ga-oxinate complex for white blood cell labeling were determined. Gallium-۶۷ was produced at AMIRS ۳۰MeV cyclotron via ۶۸Zn(p,۲n)۶۷Ga reaction in the from of ۶۷GaCl۳, and was used for radiolabeling of oxinate complex at optimized conditions. A mixture of ۶۷GaCl۳ (۳uL, ۲۰۰uCi) and ethanolic oxine solution (۱mg/ml, ۱۰۰μl) was evaporated and reacted at ۲۵ºC for ۱ h in the presence of NaOAc solution (pH. ۵.۵). ITLC was performed using a mixture of ammonium actetae and methanol solution (۱:۱) followed by recording the activity using radio thin layer chromatography scanner. The radiochemical purity of %۹۵.۱۸ at these conditions was obtained (specific activity of ۱۴۳۲ GBq/mmol). Freshly prepared white blood cells were separated from human volunteers and used for labeling by the above mentioned complex at ۳۷ºC. ۶۷Ga-oxinate complex due to it’s lipophilicity and suitable gamma rays is a suitable cell labeling agent and available for blood stem cell and microorganism studies. 

Keywords:

گالیم- ۶۷ , کمپلکس اکسینات , بهینه سازی , نشان دارسازی سلولی