Anti-cancer effects of MYC decoy Oligodeoxynucleotide-encapsulated Niosomes-Selenium hybrid nanostructure combination with X-irradiation on breast cancer cell line

Introduction: Triple negative breast cancer (TNBC) is a malignant cancer and MYC gene is considered as important oncogene in this cancer. Decoy oligodeoxynucleotides (ODNs) strategy can be used to inhibit MYC TF. In this study, we investigated the synergistic effects of MYC decoy oligodeoxynucleotides-encapsulated Niosome-selenium hybrid nanocarriers on X‐irradiation exposure as a combinational therapy on MDA-MB-۴۶۸ breast cancer cells. Methods: Decoy ODNs for MYC TF designed and synthesized based on Bcl-۲ gene promoter. Selenium nanoparticles (SeNPs) and MYC decoy ODNs encapsulated in Niosome nanosystems (NISM@Chi-Se-DEC) and FT-IR, DLS, FESEM and hemolysis tests were applied to confirm its physicochemical properties. Further investigations were carried out using uptake, cell viability, apoptosis, cell cycle, and scratch repair tests. All above assays were performed at with and without X-rays (fractionated ۲Gy) exposure conditions. Results: Physicochemical properties of Niosome nanosystems containing SeNPs and MYC decoy ODNs showed that its synthesized correctly. Uptake assay showed efficiently taken up of nanosystem by cells. NISM@Chi-Se-DEC significantly inhibited cell growth, reduced cell migration, increased apoptosis, and arrested cell cycle phases in both with and without X-rays exposure conditions. Conclusion: According to physicochemical characteristics and anti-cancer effects of nanosystem, MYC decoy Oligodeoxynucleotide-encapsulated Niosomes-Selenium hybrid nanostructure could be considered as a potential therapeutic tool along with current remedies such as radiation therapy in cancer treatment.

Keywords:

Triple negative breast cancer (TNBC) , Decoy oligodeoxynucleotides (ODNs) , MYC transcription factor , Niosome nanosystems , Combination therapy , Radiation therapy