Biosynthesis of Zn nanoparticles and in situ their hybrid with BSA nanoparticles as a Nanocarrier for Baicalein and investigation of its anti-cancer effects on U۸۷ cell line

Introduction: Glioblastoma (GBM) is the most common primary malignant brain tumor and the most aggressive malignancy to date. Baikalin (Ba) is also one of the most important flavonoids that has had several biological effects including antiviral, anti-hepatic, anti-inflammatory and anti-tumor effects. By making biocompatible and biodegradable nanosystems, nanomedicines are able to deliver conventional chemotherapy drugs in a targeted manner in vivo environment. Zinc, one of the favorite nanoparticles in medicine, has photocatalytic ability and high oxidation ability against prokaryotic and eukaryotic cells and by inducing apoptosis in tumor cells is also a cancer inhibitor.Materials and Methods:In this study Zn@BSA hybrid nanoparticles were prepared and used as carriers for Baicalein anticancer drug delivery. Zn@BSA NPs were synthesized by a single-step reduction process, and the successful preparation was verified through a list of physical characterizations, including SEM, EDX, UV spectroscopy, DLS and FT-IR.Results:Our results revealed that the stable Zn@BSA@Ba NPs was successfully synthesized with a narrow size distribution with proper zeta-potential values and had an appropriate biocompatibility. The drug release from the Zn@BSA@Ba NPs exhibited controlled and pH-dependent behavior as the best models to explain the drug release profile. Zn@BSA@Ba NPs was efficiently taken up by human glioblastoma cells (U۸۷) and significantly inhibited cell growth. Finally, delivery of Baicalein by Zn@BSA@Ba NPs resulted in, apoptosis and cell death.Conclusion:It can be concluded that the presented nanocarrier system can be a promising approach for targeted drug delivery in cancer therapy.

Keywords:

BSANPs , Drug Delivery , Zn Nanoparticles , Baicalein , U۸۷ Cell Line