Investigation of Harmine and Harmalin Alkaloids in Seganum Peganum harmala L. Seed Using Fluorimeter Technique

Peganum harmala L. is a local herb of the Nitrariaceae family found in Central Asia, the Middle East and North Africa. (۱ and ۲) Harmine and harmalin is an active drug secondary metabolite produced by Peganum harmala. (۳) which has anti-tumor effects, vasodilator effects, anti-HIV, antioxidant activity, immune system modulating properties and hypoglycemic effects.In this study, ۲۰ g of pecan seeds ground in ۳۰% (v / v) acetic acid solution were mixed for ۱۰ minutes and mixed well. The mixture was passed through ۰.۴۵ nm filter paper. Washed again with acetic acid. Then petroleum ether and ethyl acetate were added in ۱۰۰ ml and brought to pH = ۸. Finally, chloroform was added to the above solution. The obtained alkaloids were collected in the chloroform phase after evaporation. (۲), and was studied using fluorimetric technique to detect harmin and harmalins in Pecan seed extract for purification and its use as an effective substance for the expression of genes involved in HCT-۱۱۶ cancer cells. The results obtained in the readings in the wavelength range of ۳۷۶ to ۴۷۸ nm showed that the extraction method with chloroform overlaps with about ۹۰% of the standard material and can be used for loading on nano-graphene in future studies. Use the contract.

Keywords:

Peganum harmala L. , Fluorimetric technique , Purification