Investigating the Association of rs1617640 Polymorphism in Patients with Diabetes Type II and Its Complications

The prevalence of diabetes – especially diabetes type II- is increasing steadily; according to WHO reports it will increase to 366 millionpeople by the year 2030. Microvascular complications including Proliferative diabetic retinopathy (PDR) and end stage renaldisease (ESRD) are increased in patients with diabetes mellitus. Case-control association studies have demonstrated that rs1617640SNP in the promoter of erythropoietin (EPO) gene is significantly associated with PDR and ESRD. In the mentioned SNP, TT genotypeis considered as risk genotype which means that EPO concentration in human vitreous body in these people shall be higher.People with TT genotype are much more at risk of retinopathies. In this study we investigated the existence of rs1617640 EPO genepolymorphism among 150 healthy subjects and 150 subjects with diabetes type II who referred to Yazd central laboratory. Then theassociation of rs1617640 SNP with complications among diabetic patients were examined by ARMS-PCR method. The results wereanalyzed using GraphPad software (version 5.00). Prevalence of genotype GG was 8% in patients and 1.3% in the control group. GTwas 51.3% in patients, and 86.7% in the control group, and finally TT was observed in 40.7% in patients, and 12% of control group. TheTT genotype was 37.6% in patients with retinopathy and 42.6% in non-retinopathy patients. Our study demonstrates that the prevalenceof rs1617640 SNP has significant difference between diabetic patients and control group; whereas there was not any significantrelationship between this polymorphism and the complications of diabetes in patients. Together our study reveals that rs1617640SNP may be associated with susceptibility to diabetes type II; however it seems that this polymorphism is not significantly relatedto the diabetic complications in Yazd.

Investigating the Association of rs1617640 Polymorphism in Patients with Diabetes Type II and Its Complications Keywords:

Diabetes Type II , Retinopathy , Nephropathy , Neuropathy , EPO rs1617640 SNP