Investigation of phylogenetic relationships and genetic diversity of population (Seidlitzia rosmarinus) by RAPD marker in some regions of Iran
Publish place: Botanical Journal of Iran، Vol: 23، Issue: 1
Publish Year: 1401
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_ROST-23-1_006
تاریخ نمایه سازی: 23 مهر 1401
Abstract:
The genus Sedlitzia (Amaranthaceae) includes four herbaceous and one shrubby species. Sedlitzia rosmarinus is resistant to salinity that plays an important role in soil conservation. In this research, RAPD molecular markers were used to evaluate the genetic diversity among ۸۵ plant samples from ۱۷ populations of S. rosmarinus. Five primers were used to perform RAPD reactions and a total of ۴۰۰ DNA bands were obtained. After forming ۰ and ۱ matrix with the help of GenAlex ۶.۴۱ and UPGMA methods using SHAN clustering model, NTSYS Ver. ۲.۰۲ software was used to interpret RAPD analysis data. Molecular analysis of variance (AMOVA) was performed to estimate genetic diversity out of ۸۵ individuals. Out of the total diversity, it was calculated that, the highest amount of genetic diversity i.e. ۶۹% was related to intra-population and ۳۱% to inter-population diversity, respectively.
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Authors
شیما صدیقی
PhD Student, Science and Research Branch, Islamic Azad University, Tehran, Iran
علیرضا ایرانبخش
Prof., Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
سید محمد مهدی حمدی
Associate Prof., Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
ایرج مهرگان
Associate Prof., Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
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