Angiogenesis and Tumor-Associated Macrophages in Different Grades of Oral Squamous Cell Carcinoma, Verrucous Carcinoma and Epithelial Dysplasia via Immunohistochemical Assessment of Expression of CD۳۴ and CD۶۸ Markers

Background:Squamous cell carcinoma (SCC) is the most common malignancy of the oral cavity, which is highly invasive. Verrucous carcinoma (VC) is the low-grade form of SCC. Epithelial dysplasia (ED) also has a malignant potential. This immunohistochemical (IHC) study aimed to assess angiogenesis and the presence of tumor-associated macrophages (TAMs) in SCC, VC and ED to determine the role of these factors in the progression of dysplastic lesions to neoplasia. Methods:Two ۴ µ-thick sections were made of ۴۳ paraffin blocks (۱۴ SCC, ۱۴ VC and ۱۵ ED lesions confirmed by two pathologists) for IHC staining. The mean microvessel density (MVD) and the number of TAMs were determined by assessing the expression of CD۳۴ and CD۶۸, respectively in each group of lesions. Data were analyzed using the Fisher’s Exact Test, Chi-square, Kruskal Wallis tests and one-way ANOVA with the aid of SPSS version ۲۱. Results:Expression of CD۳۴ in ED was higher than that in VCand SCC (ED>VC>SCC). Expression of this marker in more severe forms of ED was higher than that in mild forms. This expression was lower in high-grade SCC in comparison to low-grade SCC. Expression of CD۶۸ in SCC was slightly higher than that in VC and ED. Expression of this marker in severe ED was less than that in mild ED. Its expression in high-grade SCC was higher than that in low-grade SCC, but these differences were not significant for CD۶۸. No significant association was noted between the expression of CD۶۸ and CD۳۴ in these lesions. Conclusion:The increase in the number of TAMs in malignant oral epithelial lesions was related to the type of tumor and its histopathological grade, but no association was found between TAMs and MVD.

Keywords:

Angiogenesis , macrophage , Squamous Cell Carcinoma , CD۳۴ , CD۶۸